Journal: Biochemistry and Biophysics Reports
Article Title: Ectopic endometrial mesenchymal stem cell-derived exosomal miR-4466 promotes angiogenesis by targeting RUNX1 in adenomyosis
doi: 10.1016/j.bbrep.2026.102457
Figure Lengend Snippet: miR-4466 directly regulates RUNX1 expression in HUVECs. (A) A Venn diagram shows target genes of miR-4466 predicted by the miRDB, miRWalk, miRPathDB, mirDIP databases. (B) Schematic diagram illustrating the predicted miR-4466 binding sites with the 3′ UTR of RUNX1. (C, D) Relative expression of target genes was detected by qRT-PCR in HUVECs (C) and HEK-293T cells (D) transfected with NC-mimics and miR-mimics. n = 3. E, F Relative expression of target genes was detected by qRT-PCR in HUVECs (E) and HEK-293T cells (F) transfected with NC-inhibitor and miR-inhibitor. n = 3. (G) Schematic representation of the wild-type (Wt) and mutant-type (Mut) binding site between the 3′ UTR of RUNX1 and miR-4466. (H) Relative luciferase activity of reporter constructs containing miRNA binding sites transfected with NC-mimics and miR-mimics in HUVECs. n = 3. (I) Western blot of RUNX1 and VEGFA protein expression in HUVECs after transfection with NC-mimics/inhibitor or miR-mimics/inhibitor for 48 h (Full-length blot are presented in Supplementary Figure). (J, K) Protein levels of RUNX1 (J) and VEGFA (K) were normalized to GAPDH protein expression level in (I). ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001 and ns: P > 0.05.
Article Snippet: Extracellular VEGFA levels in the supernatants of HUVECs and HEK-293T cells were quantified using the Human VEGFA ELISA Kit (Elabscience, TX, USA) according to the manufacturer's instructions.
Techniques: Expressing, Binding Assay, Quantitative RT-PCR, Transfection, Mutagenesis, Luciferase, Activity Assay, Construct, Western Blot
